{"id":8795,"date":"2021-03-01T12:51:17","date_gmt":"2021-03-01T12:51:17","guid":{"rendered":"https:\/\/corpsite.staging.3d4medical.cloud\/uncategorized\/preparing-a-histology-slide"},"modified":"2021-05-13T12:34:20","modified_gmt":"2021-05-13T12:34:20","slug":"preparing-a-histology-slide","status":"publish","type":"post","link":"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/blog\/preparing-a-histology-slide","title":{"rendered":"Preparing a histology slide"},"content":{"rendered":"\n<p>Histology is the microscopic study of tissues and organs achieved by staining, sectioning, and viewing those sections under a microscope ?. Histology is used in scientific study, medical diagnosis, autopsy, and even in the study of fossils ?.<\/p>\n\n\n\n<p>The four main human tissue types, epithelium, connective tissue, muscle tissue, and nervous tissue, can be viewed and distinguished with varying histological stains. There are a number of steps required before a slide can be viewed under the microscope.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Fixation<\/h2>\n\n\n\n<p>The fixation process preserves the structure of the tissue sample and prevents it from degradation by introducing chemicals that stimulate cross-linkage between proteins ?.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Dehydration<\/h2>\n\n\n\n<p>Ethanol is added to achieve dehydration of a sample ?. After the tissue sample is dehydrated, xylene can be used to remove the ethanol.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Embedding<\/h2>\n\n\n\n<p>During this phase, the tissue samples are embedded in a solid material, which allows for thin sectioning in the next stage. Paraffin wax or plastic resin are often used at this stage.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Sectioning<\/h2>\n\n\n\n<p>To section the specimen, it is placed on a precision cutting tool known as a microtome. The tissue is cut into sections of about 4-5 micrometers, which is the optimal thickness for staining.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Staining<\/h2>\n\n\n\n<p>The appropriate stain is chosen based on its unique properties. For example, Hematoxylin and Eosin are combined in H&amp;E staining. Cell nuclei are stained blue because Hematoxylin binds to DNA, which is acidic. The cytoplasm is stained pink because Eosin binds to proteins in the cytoplasm, which are basic.<\/p>\n\n\n\n<p><strong>You can explore the microscopic anatomy of the human body in our <a href=\"https:\/\/completeanatomy.cn\/apps\/complete-anatomy\/courses\/histology-of-the-human-organs\" target=\"_blank\" rel=\"noreferrer noopener\">Histology of the Human Organs Course<\/a>, available in Complete Anatomy.<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Histology is the microscopic study of tissues and organs achieved by staining, sectioning, and viewing those sections under a microscope ?. Histology is used in scientific study, medical diagnosis, autopsy, and even in the study of fossils ?.<a class=\"more-link\" href=\"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/blog\/preparing-a-histology-slide\">\u9605\u8bfb\u8be6\u60c5<\/a><\/p>\n","protected":false},"author":14,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[316],"tags":[],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/wp-json\/wp\/v2\/posts\/8795"}],"collection":[{"href":"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/wp-json\/wp\/v2\/users\/14"}],"replies":[{"embeddable":true,"href":"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/wp-json\/wp\/v2\/comments?post=8795"}],"version-history":[{"count":1,"href":"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/wp-json\/wp\/v2\/posts\/8795\/revisions"}],"predecessor-version":[{"id":9430,"href":"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/wp-json\/wp\/v2\/posts\/8795\/revisions\/9430"}],"wp:attachment":[{"href":"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/wp-json\/wp\/v2\/media?parent=8795"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/wp-json\/wp\/v2\/categories?post=8795"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/corpsite.nonprod.completeanatomy.cn\/zh-hans\/wp-json\/wp\/v2\/tags?post=8795"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}